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Bacterial DNA in mRNA vaccines: its epigenetics and incomplete linearization

M
Merogenomics
Released: 4 days ago
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What happens when bacterially methylated plasmid DNA meets the human innate immune system?
This video explores sequencing data from Pfizer lot FL8095, showing residual plasmid DNA bearing bacterial epigenetic marks — and what that could mean for immune signaling, inflammation, and future manufacturing standards.

⏱️ Timestamps:
00:00 – Intro and video title
00:24 – Bacterial DNA contamination background
07:25 – Bacterial plasmid DNA fragments size distribution
09:15 – Methylation pattern of bacterial plasmid fragments
11:39 – Non-linearization of the plasmid and its implications

📍 Filming Location: Isaac's Wet Dream Waterfall, Alberta

📄 Described content:
https://zenodo.org/records/17281691

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#mRNA #PlasmidDNA #Epigenetics #cGAS #STINGPathway #VaccineScience #Immunology #Biotech #Nanopore #DNASequencing #InnateImmunity #Methylation #MolecularBiology #SV40 #Pfizer
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